sequence fragments of the heavy chain and light chain Search Results


l9 heavy and light chain sequences  (GenScript corporation)
90
GenScript corporation l9 heavy and light chain sequences
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
L9 Heavy And Light Chain Sequences, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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heavy- and light-chain variable region sequences  (LakePharma)
90
LakePharma heavy- and light-chain variable region sequences
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Heavy And Light Chain Variable Region Sequences, supplied by LakePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequencing of heavy and light chain variable regions  (Microsynth ag)
90
Microsynth ag sequencing of heavy and light chain variable regions
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Sequencing Of Heavy And Light Chain Variable Regions, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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double stranded dna fragments coding for the light chain and heavy chain cdr sequences  (BioAtla Inc)
90
BioAtla Inc double stranded dna fragments coding for the light chain and heavy chain cdr sequences
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Double Stranded Dna Fragments Coding For The Light Chain And Heavy Chain Cdr Sequences, supplied by BioAtla Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genes encoding humanized v-region heavy (vh) and light (kappa, vk) chain sequences  (Entelechon GmbH)
90
Entelechon GmbH genes encoding humanized v-region heavy (vh) and light (kappa, vk) chain sequences
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Genes Encoding Humanized V Region Heavy (Vh) And Light (Kappa, Vk) Chain Sequences, supplied by Entelechon GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody heavy and light chains sequencing  (Microsynth ag)
90
Microsynth ag antibody heavy and light chains sequencing
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Antibody Heavy And Light Chains Sequencing, supplied by Microsynth ag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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heavy and light chains sequencing  (GenScript corporation)
90
GenScript corporation heavy and light chains sequencing
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Heavy And Light Chains Sequencing, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chikv fab cap101a.e8 variable heavy and light chain cdnas bearing human il-2 signal sequences  (GenScript corporation)
90
GenScript corporation chikv fab cap101a.e8 variable heavy and light chain cdnas bearing human il-2 signal sequences
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Chikv Fab Cap101a.E8 Variable Heavy And Light Chain Cdnas Bearing Human Il 2 Signal Sequences, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sequences of the variable regions of the heavy and light chains  (AbbVie Inc)
90
AbbVie Inc sequences of the variable regions of the heavy and light chains
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Sequences Of The Variable Regions Of The Heavy And Light Chains, supplied by AbbVie Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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full-length dna sequences of the heavy chain and beta 2 microglobulin chain (b2m) of human fcrn  (GenScript corporation)
90
GenScript corporation full-length dna sequences of the heavy chain and beta 2 microglobulin chain (b2m) of human fcrn
a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the <t>L9</t> <t>heavy</t> chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.
Full Length Dna Sequences Of The Heavy Chain And Beta 2 Microglobulin Chain (B2m) Of Human Fcrn, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hybridoma heavy and light chain variable sequences and imgt analysis  (GenScript corporation)
90
GenScript corporation hybridoma heavy and light chain variable sequences and imgt analysis
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Hybridoma Heavy And Light Chain Variable Sequences And Imgt Analysis, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mab 2e2 heavy and light chain cloning and cdna sequencing work  (Vybion Inc)
90
Vybion Inc mab 2e2 heavy and light chain cloning and cdna sequencing work
Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from <t>hybridoma</t> supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="250" height="auto" />
Mab 2e2 Heavy And Light Chain Cloning And Cdna Sequencing Work, supplied by Vybion Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the L9 heavy chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Structural basis of epitope selectivity and potent protection from malaria by PfCSP antibody L9

doi: 10.1038/s41467-023-38509-2

Figure Lengend Snippet: a Buried surface area (BSA) contributions of individual residues to rsCSP binding in the L9 heavy chain. Sequence alignment to the IGHV3-33 germline gene shown below. b Same as in a, for the L9 light chain. c , d Structural details of NPNV binding. e NPNA 2 epitope structure in the NPNA-specific mAb 2243 (PBD 6O23), highlighting the two key CH-π interactions of germline-encoded aromatic residues (W52 H and Y94 L ) with the repeat prolines. f Same as in e, with X-ray structures of six NPNA-specific mAbs superimposed to highlight structural conservation. These six mAbs are shown in g . g Electrostatic surface potentials from L9 cryo-EM structure +/− peptide (upper left two panels) and X-ray structures of six other NPNA-specific mAbs bound to peptide; electrostatic potentials were calculated in PyMol . The PDB accession codes are in parentheses. K b : Boltzmann constant; T : temperature in kelvin; c e : electron charge in coulombs. Source data are provided as a Source Data file.

Article Snippet: L9 heavy and light chain sequences were synthesized and codon-optimized for mammalian expression and cloned into pHCMV3 by Genscript Inc.

Techniques: Binding Assay, Sequencing, Cryo-EM Sample Prep

a Ribbon diagram of Fab B (cyan) and C (maroon); side chains of interacting residues are shown. b – d Structural details of key homotypic interactions. Dashed lines indicate specific contacts. e Buried surface area (BSA) contributions of individual residues to the homotypic interface in L9 light chain. Sequence alignment with F10 K and germline IGKV1-5 gene is shown below. f Same as in e , for L9 heavy chain, with sequence alignment to F10 H and germline IGHV3-33 gene. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Structural basis of epitope selectivity and potent protection from malaria by PfCSP antibody L9

doi: 10.1038/s41467-023-38509-2

Figure Lengend Snippet: a Ribbon diagram of Fab B (cyan) and C (maroon); side chains of interacting residues are shown. b – d Structural details of key homotypic interactions. Dashed lines indicate specific contacts. e Buried surface area (BSA) contributions of individual residues to the homotypic interface in L9 light chain. Sequence alignment with F10 K and germline IGKV1-5 gene is shown below. f Same as in e , for L9 heavy chain, with sequence alignment to F10 H and germline IGHV3-33 gene. Source data are provided as a Source Data file.

Article Snippet: L9 heavy and light chain sequences were synthesized and codon-optimized for mammalian expression and cloned into pHCMV3 by Genscript Inc.

Techniques: Sequencing

Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from hybridoma supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also <xref ref-type=Figure S1 . " width="100%" height="100%">

Journal: Structure (London, England : 1993)

Article Title: Vaccine-elicited murine antibody WS6 neutralizes diverse beta-coronaviruses by recognizing a helical stem supersite of vulnerability

doi: 10.1016/j.str.2022.06.004

Figure Lengend Snippet: Spike-mRNA-immunized mice elicit antibodies against diverse regions of spike, several of which bound diverse beta-coronavirus spikes and one of which, WS6, neutralized them (A) Immunization scheme. NatSP is the full transmembrane-containing native sequence of spike WA-1 strain; S-dTM is the soluble spike protein residues 1–1,206 of wild-type WA-1 strain. (B) Binding of isolated antibodies by ELISA to subdomains of the SARS-CoV-2 spike. Purified monoclonal antibodies from hybridoma supernatants were analyzed for binding to SARS-CoV-2 S-dTM, S1 (S1-short and S1R), RBD, and NTD by ELISA. (C) Binding of isolated antibodies assessed by ELISA to diverse beta-coronavirus pre-fusion-stabilized spikes (S2Ps). (D) Neutralization assessment of hybridoma antibodies against SARS-CoV-2 WA-1 and SARS-CoV pseudoviruses on 293T-ACE2 cells. See also Figure S1 .

Article Snippet: Select hybridomas were sent to GenScript (Piscataway, NJ 08854, USA) for hybridoma heavy and light chain variable sequences and IMGT ( ) analysis.

Techniques: Sequencing, Binding Assay, Isolation, Enzyme-linked Immunosorbent Assay, Purification, Bioprocessing, Neutralization